Rabbit Anti-phospho-NFATc2 (Ser330) antibody |
反应物种(预测) |
Dog,Pig,Cow,Horse |
产品应用(已验证) |
WB,IHC,FCM |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=1ug/Test, |
研究领域 |
免疫学,神经生物学,信号转导,转录调节因子,激酶和磷酸酶,T-淋巴细胞, |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-NFATc2(Ser330)) Polyclonal Antibody, Unconjugated (bs-5516R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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Sample:
Raji Cell (Human) Lysate at 30 ug
Primary: Anti- p-NFATc2(Ser330) (bs-5516R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100kD
Observed band size: 110 kD
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Sample:
Spleen (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- p-NFATc2(Ser330) (bs-5516R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100kD
Observed band size: 110 kD
RRID:AB_11081901
产品名称:Rabbit Anti-phospho-NFATc2 (Ser330) antibody
别名: NFATC2(phospho Ser330); NFATC2(phospho S330); AI607462; KIAA0611; NF ATp; NF-ATc2; NFAT 1; NFAT pre existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1-D; NFATc2; NFATp; Nuclear factor of activated T cells cytoplasmic 2; Nucl
中文名称:磷酸化核因子活化T细胞胞浆蛋白2抗体
英文名称:Rabbit Anti-phospho-NFATc2 (Ser330) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human NFATc2 around the phosphorylation site of Ser330
抗原表位:DP(p-S)PV
SWISS:Q13469
Gene ID :4773
Human Gene ID:4773
This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]
Function:Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.
Subunit:Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating pro
Subcellular Location:Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between susta
Tissue Specificity:Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
Post-translational modifications:In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-365 region. Upon cell stimulation, all these sites except Ser-245 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously ex
Similarity:Contains 1 RHD (Rel-like) domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.