Rabbit Anti-phospho-SNAIL + SLUG (Ser246) antibody |
反应物种(预测) |
Mouse,Rat,Horse,Rabbit |
产品应用(已验证) |
FCM |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,Flow Cyt=1μg/Test, |
研究领域 |
肿瘤,细胞生物,免疫学,发育生物学,神经生物学,信号转导,干细胞,细胞凋亡,锌指蛋白,表观遗传学, |
标签 |
Array |
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Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti- phospho-SNAIL + SLUG (Ser246)/AF488 antibody(bs-11962R-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
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Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-phospho-SNAIL + SLUG (Ser246) antibody (bs-11962R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:RRID
产品名称:Rabbit Anti-phospho-SNAIL + SLUG (Ser246) antibody
别名: SNAIL + SLUG (phospho S246); p-SNAIL + SLUG (phospho S246); SNAIL + SLUG (phospho S246 + S251); SNAIL + SLUG (phospho Ser246 + Ser251); phospho-SNAIL(Ser246) + SLUG(Ser251); phospho-SNAIL(Ser246) + SLUG(Ser251); dJ710H13.1; MGC10182; Neural crest transcr
中文名称:磷酸化锌指转录因子Slug+SNAIL抗体
英文名称:Rabbit Anti-phospho-SNAIL + SLUG (Ser246) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthesised phosphopeptide derived from human SNAIL around the phosphorylation site of Ser246
抗原表位:TF(p-S)RM
SWISS:O95863
Gene ID :6615
Human Gene ID:6615
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin. SLUG is involved in epithelial-mesenchymal transitions (EMT) involving E-cadherin repression which is known to play an important role in tumor progression amd neural tube formation. SLUG also has antiapoptotic activity. Mutations in this gene may be associated with sporadic cases of neural tube defects (referenced from Entrez gene).
Function:SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional represso
Subunit:Interacts with FBXL14 and GSK3B. Interacts with BTRC; interaction occurs when it is phosphorylated on the destruction motif. Interacts (via SNAG domain) with WTIP (via LIM domains) (By similarity). Interacts (via SNAG domain) with LIMD1 (via LIM domains),
Subcellular Location:Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
Tissue Specificity:Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
Post-translational modifications:Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 ph
Similarity:Belongs to the snail C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.