| Rabbit Anti-phospho-beta Actin (Tyr53) antibody |
| 反应物种（预测） |
Chicken,Pig,Cow,Rabbit |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,ICC=1:100, |
| 研究领域 |
细胞生物,信号转导,细胞骨架, |
| 标签 |
Array |
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Blank control: NIH/3T3.
Primary Antibody (green line): Rabbit Anti-phospho-beta Actin (Tyr53) antibody (bs-12581R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Lane 3: Jurkat (Human) Cell Lysate at 30 ug
Primary:
Anti- phospho-beta Actin (Tyr53) (bs-12581R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta Actin (Tyr53)) Polyclonal Antibody, Unconjugated (bs-12581R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti- phospho-beta Actin (Tyr53) (bs-12581R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
RRID：RRID
产品名称：Rabbit Anti-phospho-beta Actin (Tyr53) antibody
别名： beta Actin (phospho Y53); p-beta Actin (phospho Y53); A26C1A; A26C1B; ACTB; ACTB_HUMAN; Actin cytoplasmic 1; Actx; beta cytoskeletal actin; Beta-actin; E430023M04Rik; MGC128179; PS1TP5 binding protein 1; PS1TP5BP1.
中文名称：磷酸化β-肌动蛋白抗体
英文名称：Rabbit Anti-phospho-beta Actin (Tyr53) antibody
中文别名：β actin; β-actin; βactin;
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human beta Actin around the phosphorylation site of Tyr53
抗原表位：DS(p-Y)VG
SWISS：P60709
Gene ID ：60
Human Gene ID：60
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Function：Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit：Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1
Subcellular Location：Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Post-translational modifications：ISGylated.
Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxi
DISEASE：Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients
Similarity：Belongs to the actin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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