| Rabbit Anti-phospho-Cytokeratin 8 (Ser73) antibody |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
WB,IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,信号转导,细胞类型标志物, |
| 标签 |
Array |
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Blank control:MCF-7.
Primary Antibody (green line): Rabbit Anti-phospho-Cytokeratin 8 (Ser73) antibody (bs-12919R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-phospho-Cytokeratin 8 (Ser73) antibody
别名： Cytokeratin 8 (phospho S73); p-Cytokeratin 8 (phospho S73); card2; Cardiac autoantigen 2 120kD; CK 8; CK-8; ck8; Cyk 8; cyk8; CYKER; Cytokeratin-8; Cytokeratin endo A; Cytokeratin-8; Cytokeratin8; DreK8; EndoA; k0; CYK8; k2c8; K2C8_HUMAN; k8; Keratin 8; K
中文名称：磷酸化细胞角蛋白8抗体
英文名称：Rabbit Anti-phospho-Cytokeratin 8 (Ser73) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human CK8 around the phosphorylation site of Ser73
抗原表位：LL(p-S)PL
SWISS：P05787
Gene ID ：3856
Human Gene ID：3856
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].
Function：Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Subunit：Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1.
Subcellular Location：Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
Tissue Specificity：Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Post-translational modifications：Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases
DISEASE：Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600]..
Similarity：Belongs to the intermediate filament family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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