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Rabbit Anti-Vimentin antibody
多克隆  |   SKU:bs-8533R

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货号:bs-3351R
¥1280
订购号:bs-8533R
¥1180.00-3200.00
货期:现货
Rabbit Anti-Vimentin antibody
反应物种(预测)

Rat,Dog,Pig,Cow,Horse

产品应用(已验证)

WB,ICC,IF,FCM

产品应用(可尝试)

IHC,ELISA

推荐稀释比例

WB=1:500-2000,Elisa=1:5000-10000,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100,

研究领域

肿瘤,细胞生物,免疫学,信号转导,干细胞,细胞骨架,肿瘤细胞生物标志物,

标签

Array

  • Sample:
    U-87MG (Human) Cell Lysate at 30 ug
    NIH/3T3 (Mouse) Cell Lysate at 30 ug
    Primary: Anti-Vimentin (bs-8533R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 53 kD
  • Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vimentin) polyclonal Antibody, Unconjugated (bs-8533R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
  • Tissue/cell: endothelial cells of umbilical artery;4% Paraformaldehyde-fixed;
    Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Vimentin Polyclonal Antibody, Alexa Fluor 488 conjugated(bs-8533R-A488) 1:100, 60 minutes at 37℃. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
  • Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
    Incubation: Anti-Vimentin Polyclonal Antibody, Unconjugated(bs-8533R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  • Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
    Incubation: Anti-Vimentin Polyclonal Antibody, Unconjugated(bs-8533R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  • Protein: spleen(mouse) lysate at 40ug;
    Primary: rabbit Anti-Vimentin (bs-8533R) at 1:300;
    Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
    Predicted band size: 51 kD
    Observed band size: 51 kD
  • Protein: lung(rabbit) lysate at 40ug;
    Primary: rabbit Anti-Vimentin (bs-8533R) at 1:300;
    Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
    Predicted band size: 51 kD
    Observed band size: 51 kD
  • Sample: lymph node (Mouse) Lysate at 40 ug
    Primary: Anti-Vimentin(bs-8533R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 51 kD
  • Sample: skin(Mouse) Lysate at 40 ug
    Primary: Anti-Vimentin(bs-8533R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 51 kD
  • Tissue/cell: FHC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-8533R) 1:200, 2 hours at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
  • Tissue/cell: 293T cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-8533R) 1:200, 2 hours at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
  • Sample:
    A549(Human) Cell Lysate at 30 ug
    HUVEC(Human) Cell Lysate at 30 ug
    U251(Human) Cell Lysate at 30 ug
    Primary: Anti-Vimentin (bs-8533R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 53 kD
    Observed band size: 53 kD
  • Blank control:A549.
    Primary Antibody (green line): Rabbit Anti-Vimentin antibody (bs-8533R)
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-AF488
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
  • Sample: Lung (Mouse) Lysate at 40 ug
    Primary: Anti-Vimentin (bs-8533R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 51kD
    Observed band size: 51kD
  • Sample:
    A549(Human) Cell Lysate at 30 ug
    Jurkat(Human) Cell Lysate at 30 ug
    Primary: Anti-Vimentin   (bs-8533R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 53 kD
  • Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-8533R)antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
  • Sample:
    Hela KO Vimentin (Human) Cell Lysate at 30 ug
    Hela(Human) Cell Lysate at 30 ug
    Primary: Anti- Vimentin (bs-8533R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 57 kD

产品信息

RRID:RRID
产品名称:Rabbit Anti-Vimentin antibody
别名: VIM; FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.
中文名称:波形蛋白抗体
英文名称:Rabbit Anti-Vimentin antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated synthetic peptide derived from human Vimentin
抗原表位:371-466/466
SWISS:P08670
Gene ID :7431
Human Gene ID:7431

产品介绍

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
Function:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the st
Subcellular Location:Cytoplasm.
Tissue Specificity:Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Post-translational modifications:Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin f
Similarity:Belongs to the intermediate filament family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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