Rabbit Anti-phospho-14-3-3 beta + zeta (Ser186 / Ser184) antibody |
反应物种(预测) |
Chicken,Dog,Pig,Cow,Horse |
产品应用(已验证) |
IHC,FCM |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2μg/Test,IF=1:100-500, |
研究领域 |
细胞生物,神经生物学,信号转导,细胞凋亡 |
标签 |
Array |
-
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (YWHAB) Polyclonal Antibody, Unconjugated (bs-13773R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (YWHAB) Polyclonal Antibody, Unconjugated (bs-13773R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 beta+zeta (Ser186/Ser184) antibody (bs-13773R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-phospho-14-3-3 beta + zeta (Ser186) antibody (bs-13773R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
RRID:RRID
产品名称:Rabbit Anti-phospho-14-3-3 beta + zeta (Ser186 / Ser184) antibody
别名: 14-3-3 beta + zeta (phospho S184 + S186); 14-3-3 beta + zeta (phospho S186); 14-3-3 beta + zeta (phospho Ser186); p-14-3-3 beta + zeta (Ser186) 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta/delta; 14 3 3 zeta; HS1; KCIP1; YWHAB; YWHA
中文名称:磷酸化14-3-3 β/ζ抗体
英文名称:Rabbit Anti-phospho-14-3-3 beta + zeta (Ser186 / Ser184) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthesised phosphopeptide derived from human 14-3-3 beta + zeta around the phosphorylation site of Ser186 / Ser184
抗原表位:LN(p-S)PE
SWISS:P31946
Gene ID :7529
Human Gene ID:7529
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.
Function:14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and p
Subunit:Interacts with CDK16 and BSPRY. Interacts with WEE1 (C-terminal). Interacts with SAMSN1. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with BCL2L11. Homodimer. He
Subcellular Location:Cytoplasmic. Melanosome (fractions from stage I to stage IV)
Post-translational modifications:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homod
Similarity:Belongs to the 14-3-3 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.