Rabbit Anti-ERO1LB antibody |
反应物种(预测) |
Human,Rat,Rabbit,GuineaPig |
产品应用(已验证) |
WB |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000, |
研究领域 |
细胞生物,信号转导,转运蛋白, |
标签 |
Array |
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Sample: Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-ERO1LB (bs-14627R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Sample:
Liver (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti- ERO1LB (bs-14627R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
RRID:RRID
产品名称:Rabbit Anti-ERO1LB antibody
别名: Endoplasmic oxidoreductin-1-like protein B;
ERO1-L-beta; ERO1B_HUMAN;
ERO1-like protein beta;
Oxidoreductin-1-L-beta.
中文名称:Ero1-Lβ蛋白抗体
英文名称:Rabbit Anti-ERO1LB antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human ERO1LB
抗原表位:1-100/467
SWISS:Q86YB8
Gene ID :56605
Human Gene ID:56605
Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress.
Function:Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein
Subunit:Homodimer; disulfide-linked. Heterodimer with ERO1L; disulfide-linked. Also detected as monomer. Homodimers may be somewhat less active than monomers. Interacts with P4HB. Interacts with ERP44.
Subcellular Location:Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side. Note: The association with ERP44 may be essential for its retention in the endoplasmic reticulum.
Tissue Specificity:Highly expressed in the digestive tract, including the duodenum and lower digestive tract. In the stomach, highly expressed in enzyme-producing chief cells (at protein level). In the pancreas, expressed in islets of Langerhans and, at lower levels, in enz
Post-translational modifications:N-glycosylated.
The Cys-90/Cys-95 and Cys-393/Cys-396 disulfide bonds constitute the redox-active center. The Cys-90/Cys-95 disulfide bond accepts electron from P4HB and funnel them to the active site disulfide Cys-393/Cys-396. The Cys-81/Cys-390 disul
Similarity:Belongs to the EROs family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.