| Rabbit Anti-phospho-Integrin beta 1 (Tyr783) antibody |
| 反应物种（预测） |
Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100, |
| 研究领域 |
细胞生物,神经生物学,信号转导,干细胞 |
| 标签 |
Array |
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Blank control (blue line): Hela (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
Primary Antibody (green line): Rabbit Anti-phospho-Integrin beta 1(Tyr783) antibody (bs-16635R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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Blank control(blue): U251 (fixed with Ice-cold 70% ethanol overnight at 4 °C).
Primary Antibody:Rabbit Anti-phospho-Integrin beta 1(Tyr783) antibody (bs-16635R,Green); Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Lane 3: U251 (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-Integrin beta 1 (Tyr783) (bs-16635R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
RRID：RRID
产品名称：Rabbit Anti-phospho-Integrin beta 1 (Tyr783) antibody
别名： Integrin beta 1 (phospho Y783); p-Integrin beta 1 (phospho Tyr783); Integrin beta-1; beta 1 subunit; CD 29; CD-29; CD29; CD29 antigen; Fibrinogen Receptor beta subunit; Fibronectin receptor subunit beta; Fibronectin receptor, beta subunit; FNRB; GPIIA; GP
中文名称：磷酸化整合素β1（CD29）抗体
英文名称：Rabbit Anti-phospho-Integrin beta 1 (Tyr783) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human Integrin beta 1 around the phosphorylation site of Tyr783
抗原表位：NPI(p-Y)KS
SWISS：P05556
Gene ID ：3688
Human Gene ID：3688
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function：Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/be
Subcellular Location：Cell membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection > lamellipodium. Cell projection > ruffle. Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neurobla
Tissue Specificity：Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cell
Post-translational modifications：The cysteine residues are involved in intrachain disulfide bonds.
Similarity：Belongs to the integrin beta chain family.
Contains 1 VWFA domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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