Rabbit Anti-LXR alpha + LXR beta antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-LXR alpha + LXR beta antibody

多克隆 | SKU：bs-18451R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-18451R

￥1138.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-LXR alpha + LXR beta antibody
反应物种（预测）	Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Xenopus laevis
产品应用（已验证）	WB,FCM
产品应用（可尝试）	IHC,ICC,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500,ICC=1:100-500,
研究领域	肿瘤,细胞生物,信号转导,细胞膜受体,表观遗传学,
标签	Array

Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-LXR alpha + LXR beta antibody (bs-18451R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-LXR alpha + LXR beta (bs-18451R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

产品信息

RRID：RRID
产品名称：Rabbit Anti-LXR alpha + LXR beta antibody
别名： Liver X receptor alpha; Liver X receptor alpha; Liver X receptor beta; LXR a; LXR; LXR b; LXRa; LXRalpha; LXRb; LXRbeta; NER; NERI; NR1H2; NR1H3; Nuclear orphan receptor LXR alpha; Nuclear orphan receptor LXR beta; Nuclear receptor NER; N
中文名称：肝脏X受体α+肝脏X受体β抗体
英文名称：Rabbit Anti-LXR alpha + LXR beta antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human LXR alpha
抗原表位：51-150/447
SWISS：Q13133
Gene ID ：10062
Human Gene ID：10062

产品介绍

LXR alpha and LXR beta are orphan receptors. Interaction with RXR shifts RXR from its role as a silent DNA binding partner to an active ligand binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4 type response elements characterized by direct repeats of two similar hexanuclotide half sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis. LXR alpha: Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. LXR beta: Ubiquitously expressed.
Function：Nuclear receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and L
Subcellular Location：Nuclear
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.