| Rabbit Anti-phospho-NEUROD1 (Ser274) antibody |
| 反应物种（预测） |
Pig,Cow,Sheep |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
肿瘤,心血管,细胞生物,神经生物学,表观遗传学, |
| 标签 |
Array |
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Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-phospho-NeuroD1 (Ser274) (bs-19218R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
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Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuroD1 (Ser274)) Polyclonal Antibody, Unconjugated (bs-19218R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuroD1 (Ser274)) Polyclonal Antibody, Unconjugated (bs-19218R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuroD1 (Ser274)) Polyclonal Antibody, Unconjugated (bs-19218R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：RRID
产品名称：Rabbit Anti-phospho-NEUROD1 (Ser274) antibody
别名： NeuroD1 (phospho S274); p-NeuroD1 (phospho S274);
atonal;
basic helix loop helix transcription factor;
BETA 2;
Beta cell E box transactivator 2;
BETA2;
BHF 1;
BHF1;
bHLHa3;
class A basic helix loop helix protein 3;
Class A basic helix-loop-helix
中文名称：磷酸化神经细胞分化因子1抗体
英文名称：Rabbit Anti-phospho-NEUROD1 (Ser274) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised acetylpeptide derived from human NEUROD1 around the acetylation site of Ser274
抗原表位：PL(p-S)PP
SWISS：Q13562
Gene ID ：4760
Human Gene ID：4760
This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]
Function：Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
Subcellular Location：Cytoplasm. Nucleus.
Post-translational modifications：Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
DISEASE：Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 y
Similarity：Contains 1 basic helix-loop-helix (bHLH) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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