Rabbit Anti-PSMD14 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-PSMD14 antibody

多克隆 | SKU：bs-19469R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-19469R

￥1138.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-PSMD14 antibody
反应物种（预测）	Chicken,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep
产品应用（已验证）	WB,IHC
产品应用（可尝试）	ICC,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
研究领域	细胞生物,转录调节因子,表观遗传学,泛素,
标签	Array

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (bs-19469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NTF2) Polyclonal Antibody, Unconjugated (bs-19496R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (bs-19469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (bs-19469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (bs-19469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (bs-19469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMD14) Polyclonal Antibody, Unconjugated (bs-19469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: Raw264.7 (Mouse) Lysate at 40 ug
Lane 4: Testis (Rat) Lysate at 40 ug
Lane 5: Cerebrum (Rat) Lysate at 40 ug
Lane 6: Hela (Human) Cell Lysate at 30 ug
Lane 7: U2os (Human) Cell Lysate at 30 ug
Lane 8: K562 (Human) Cell Lysate at 30 ug
Primary:
Anti-PSMD14 (bs-19469R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD

产品信息

RRID：RRID
产品名称：Rabbit Anti-PSMD14 antibody
别名： 26S proteasome non-ATPase regulatory subunit 14; 26S proteasome regulatory subunit rpn11; 26S proteasome-associated PAD1 homolog 1; 26S proteasome-associated PAD1 homolog; PAD1; PAD1, yeast, homolog of; POH1; Proteasome (prosome, macropain) 26S sub
中文名称：蛋白酶调解因子14抗体
英文名称：Rabbit Anti-PSMD14 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human PSMD14
抗原表位：221-310/310
SWISS：O00487
Gene ID ：10213
Human Gene ID：10213

产品介绍

This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012]
Function：Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitinati
Tissue Specificity：Widely expressed. Highest levels in heart and skeletal muscle.
Similarity：Belongs to the peptidase M67A family. PSMD14 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.