| Rabbit Anti-phospho-CDKN1B (Ser10) antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Rabbit,Sheep,GuineaPig |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=1μg/Test, |
| 研究领域 |
免疫学,细胞周期蛋白,激酶和磷酸酶 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CDKN1B (Ser10)) Polyclonal Antibody, Unconjugated (bs-20074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CDKN1B (Ser10)) Polyclonal Antibody, Unconjugated (bs-20074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control (blue line): MCF7(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-phospho-CDKN1B (Ser10) antibody (bs-20074R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- phospho-CDKN1B (Ser10) (bs-20074R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22kD
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Sample:
Lymphocyte (Rat) Lysate at 40 ug
Primary: Anti- phospho-CDKN1B (Ser10) (bs-20074R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22kD
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Sample:
MOLT-4 Cell (Human) Lysate at 30 ug
Primary: Anti- phospho-CDKN1B (Ser10) (bs-20074R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22kD
RRID：RRID
产品名称：Rabbit Anti-phospho-CDKN1B (Ser10) antibody
别名： p27 KIP 1 (phospho S10); p-p27 KIP 1 (phospho S10); p27Kip1(Phospho-Ser10); p-p27Kip1(Ser10); p-p27Kip1(S10); CDN1B_HUMAN; AA408329; AI843786; Cdki1b; CDKN 1B; CDKN 4; CDKN1B; CDKN1B; CDKN4; CDKN4; Cyclin Dependent Kinase Inhibitor 1B; Cyclin Dependent Ki
中文名称：磷酸化P27抗体/周期素依赖激酶抑制剂
英文名称：Rabbit Anti-phospho-CDKN1B (Ser10) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human CDKN1B around the phosphorylation site of Ser10
抗原表位：NG(p-S)PS
SWISS：P46527
Gene ID ：1027
Human Gene ID：1027
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq, Jul 2008]
Function：Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 com
Subunit：Forms a ternary compex with CCNE1/CDK2/CDKN1B.
Subcellular Location：Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-
Tissue Specificity：Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Post-translational modifications：Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other
DISEASE：Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and
Similarity：Belongs to the CDI family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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