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Rabbit Anti-SMEK1 antibody
多克隆  |   SKU:bs-12636R

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货号:bs-3351R
¥1280
订购号:bs-12636R
¥1138.00-2900.00
货期:现货
Rabbit Anti-SMEK1 antibody
反应物种(预测)

Chicken,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,GuineaPig

产品应用(已验证)

WB,IHC

产品应用(可尝试)

ICC,IF,ELISA

推荐稀释比例

WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,

研究领域

细胞生物,转录调节因子,激酶和磷酸酶,表观遗传学,

标签

Array

  • Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMEK1) Polyclonal Antibody, Unconjugated (bs-12636R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMEK1) Polyclonal Antibody, Unconjugated (bs-12636R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMEK1) Polyclonal Antibody, Unconjugated (bs-12636R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMEK1) Polyclonal Antibody, Unconjugated (bs-12636R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

产品信息

RRID:RRID
产品名称:Rabbit Anti-SMEK1 antibody
别名: FLFL1; KIAA2010; MEK1 SUPPRESSOR 1; MSTP033; P4R3A_HUMAN; PP4R3 ALPHA; PP4R3A; PPP4R3A; PROTEIN PHOSPHATASE 4, REGULATORY SUBUNIT 3, ALPHA; Serine/threonine-protein phosphatase 4 regulatory subunit 3A; SMEK homolog 1; SMEK homolog 1, suppressor
中文名称:MEK1抑制蛋白1抗体
英文名称:Rabbit Anti-SMEK1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated synthetic peptide derived from human SMEK1
抗原表位:601-700/833
SWISS:Q6IN85
Gene ID :55671
Human Gene ID:55671

产品介绍

Function:Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2A
Subunit:Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R2-PPP4R3A. Interacts with PPP4C; the interaction requires PPP4R2.
Subcellular Location:Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions.
Similarity:Belongs to the SMEK family.
Contains 1 WH1 domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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