Rabbit Anti-phospho-DNM1L (Ser616) antibody |
反应物种(预测) |
Mouse,Rat,Dog,Pig,GuineaPig |
产品应用(已验证) |
IHC,FCM |
产品应用(可尝试) |
ICC,IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,ICC=1:100-500, |
研究领域 |
肿瘤,细胞生物,神经生物学,信号转导,细胞凋亡,表观遗传学, |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-DRP1 (Ser616)) Polyclonal Antibody, Unconjugated (bs-12702R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control:U2-OS.
Primary Antibody (green line): Rabbit Anti-phospho-DNM1L (Ser616) antibody (bs-12702R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:RRID
产品名称:Rabbit Anti-phospho-DNM1L (Ser616) antibody
别名: DRP1 (phospho S616); p-DRP1 (phospho S616); P-DRP1 (Ser616); DLP1; DRP1; DNM1L_HUMAN; Dnm1p/Vps1p-like protein; DVLP; Dymple; Dynamin 1 like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin like protein; Dynamin related protein 1
中文名称:磷酸化动力相关蛋白1抗体
英文名称:Rabbit Anti-phospho-DNM1L (Ser616) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthesised phosphopeptide derived from human DRP1 around the phosphorylation site of Ser616
抗原表位:PA(p-S)PQ
SWISS:O00429
Gene ID :10059
Human Gene ID:10059
This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]
Function:Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechan
Subcellular Location:Cytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Assoc
Tissue Specificity:Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in b
Post-translational modifications:Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP
DISEASE:Note=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage.
Similarity:Belongs to the dynamin family.
Contains 1 GED domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.