| Rabbit Anti-phospho-Vimentin (Ser55) antibody |
| 反应物种（预测） |
Horse |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=2ug/Test, |
| 研究领域 |
肿瘤,细胞生物,发育生物学,神经生物学,信号转导 |
| 标签 |
Array |
-
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-phospho-Vimentin (Ser55) antibody (bs-12757R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Sample:
HT1080(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-Vimentin (Ser55) (bs-12757R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
-
Sample:
Hela(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-Vimentin (Ser55) (bs-12757R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 58 kD
RRID：RRID
产品名称：Rabbit Anti-phospho-Vimentin (Ser55) antibody
别名： Vimentin (phospho S55); p-Vimentin (phospho S55); CTRCT30;
Epididymis luminal protein 113;
FLJ36605;
HEL113;
VIM;
VIME_HUMAN;
vimentin.
中文名称：磷酸化波形蛋白抗体
英文名称：Rabbit Anti-phospho-Vimentin (Ser55) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser55
抗原表位：YA(p-S)SP
SWISS：P08670
Gene ID ：7431
Human Gene ID：7431
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
Function：Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in th
Subcellular Location：Cytoplasm.
Tissue Specificity：Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Post-translational modifications：Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which
DISEASE：Cataract 30;
The disease is caused by mutations affecting the gene represented in this entry.
Disease description:An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in mor
Similarity：Belongs to the intermediate filament family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏