Rabbit Anti-VP16 tag antibody |
反应物种(预测) |
Species independent |
产品应用(已验证) |
WB |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:1000-5000,Elisa=1:5000-10000, |
研究领域 |
免疫学,细菌及病毒, |
标签 |
Array |
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Sample:
Lane 1: VP16-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: VP16-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-VP16 tag (bs-2111R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
RRID:RRID
产品名称:Rabbit Anti-VP16 tag antibody
别名: VP16_HHV1F;UL48; Tegument protein VP16; Alpha trans-inducing protein; Alpha-TIF; ICP25; Vmw65
中文名称:VP16 tag抗体
英文名称:Rabbit Anti-VP16 tag antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from HSV-1 VP16
抗原表位:391-490/490aa
SWISS:P04486
Gene ID :VP16 tag
Human Gene ID:VP16 tag
HSV evades the immune system through interference with MHC class I antigen presentation on the cell surface, by blocking TAP or the transporter associated with antigen processing induced by the secretion of ICP-47 by HSV. In the host cell, TAP transports digested viral antigen epitope peptides from the cytosol to the endoplasmic reticulum, allowing these epitopes to be combined with MHC class I molecules and presented on the surface of the cell. Viral epitope presentation with MHC class I is a requirement for activation of cytotoxic T-lymphocytes (CTLs), the major effectors of the cell-mediated immune response against virally-infected cells. ICP-47 prevents initiation of a CTL-response against HSV, allowing the virus to survive for a protracted period in the host.
Function:Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two
Subunit:Interacts with VP22. Interacts with gH (via C-terminus). Interacts with the virion host shutoff protein (vhs). Interacts with VP11/12. Associates with the VP16-induced complex; binding to host HCFC1 activates VP16 for association with the octamer motif-bi
Similarity:Belongs to the herpesviridae tegument protein VP16 protein family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.