Mouse Anti-Aquaporin 4 antibody |
产品应用(已验证) |
IHC |
产品应用(可尝试) |
WB,ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-1500,Elisa=1:1000-5000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
肿瘤,信号转导,通道蛋白,新陈代谢, |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 4) Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 4) Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 4) Monoclonal Antibody, Unconjugated (ascites of bsm-33068M 6G1) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
RRID:RRID
产品名称:Mouse Anti-Aquaporin 4 antibody
别名: aquaporin 4; WCH4; Mercurial-insensitive water channel; MIWC; AQP 4; AQP4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; MGC22454; MIWC; AQP4_HUMAN; Aquaporin-4; AQP-4.
中文名称:水通道蛋白4单克隆抗体
英文名称:Mouse Anti-Aquaporin 4 antibody
抗体来源: Mouse
克隆类型:单克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG1
纯化方法:affinity purified by Protein G
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:Recombinant human Aquaporin 4 Protein
SWISS:P55087
Gene ID :361
Human Gene ID:361
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Subunit:Homotetramer. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1.
Subcellular Location:Membrane; Multi-pass membrane protein.
Tissue Specificity:Brain, muscle, heart, kidney, lung, and trachea.
Similarity:Belongs to the MIP/aquaporin (TC 1.A.8) family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.