Mouse Anti-Caspase 8 antibody |
反应物种(预测) |
Rat |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:2000-5000,Elisa=1:5000-10000,IHC-P=1:400-1000,IHC-F=1:500-1000,IF=1:500-1000, |
研究领域 |
细胞生物,神经生物学,细胞凋亡 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of bsm-33190M 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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Sample:
Hela(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41'43'55 kD
Observed band size: 43 kD
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Sample:
Stomach(Mouse) Lysate at 40 ug
Spleen(Mouse) Lysate at 40 ug
Lymph node(Mouse) Lysate at 40 ug
Urinary bladder(Mouse) Lysate at 40 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41'43'55 kD
Observed band size: 55'53D
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Sample:
293T-UV(Human) Cell Lysate at 30 ug
U251-UV(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase 8 (bsm-33190M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41’43’55 kD
Observed band size: 43 kD
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Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (bsm-33190M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
RRID:RRID
产品名称:Mouse Anti-Caspase 8 antibody
别名: Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CA
中文名称:半胱氨酸蛋白酶8单克隆抗体
英文名称:Mouse Anti-Caspase 8 antibody
抗体来源: Mouse
克隆类型:单克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein G
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:Recombinant human Caspase 8 Protein
SWISS:Q14790
Gene ID :841
Human Gene ID:841
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
Function:Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signa
Subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31,
Subcellular Location:Cytoplasm.
Tissue Specificity:Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
DISEASE:Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of per
Similarity:Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.