Mouse Anti-Beta catenin antibody |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
ICC,IF |
推荐稀释比例 |
WB=1:1000-5000,IHC-P=1:500-1000,IHC-F=1:500-1000,IF=1:500-1000,ICC=1:100-500, |
研究领域 |
肿瘤,细胞生物,信号转导,细胞凋亡 |
标签 |
Array |
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Sample:
U87MG(Human) Cell Lysate at 30 ug
U2os(Human) Cell Lysate at 30 ug
Primary: Anti- Beta catenin (bsm-33194M) at 1/5000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 92 kD
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Sample:
Hela(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Medulld(Mouse) Lysate at 40 ug
Testis(Mouse) Lysate at 40 ug
Cerebrum(Rat) Lysate at 40 ug
Primary: Anti-Beta catenin (bsm-33194M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 95 kD
RRID:RRID
产品名称:Mouse Anti-Beta catenin antibody
别名: beta-catenin; CHBCAT; CTNNB1; CTNNB; PRO2286; Cadherin associated protein; Catenin (cadherin associated protein), beta 1, 88kDa; Catenin beta 1; Catenin beta-1; CATNB; CTNB1_HUMAN; DKFZp686D02253; FLJ25606; FLJ37923; b-catenin; Catenin-β; Catenin β.
中文名称:β-连环蛋白/β-连环素/β链接素单克隆抗体
英文名称:Mouse Anti-Beta catenin antibody
抗体来源: Mouse
克隆类型:单克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein G
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:Recombinant human Beta catenin
SWISS:P35222
Gene ID :1499
Human Gene ID:1499
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009].
Function:Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and it
Subunit:Two separate complex-associated pools are found in the cytoplasm. The majority is present as component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and beta-catenin/CTNNB1, and possibly alpha-catenin/CTNNA1; the complex
Subcellular Location:Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Cytoplasmic when it is unstabilized (high level of phosphorylatio
Tissue Specificity:Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).
Post-translational modifications:Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-4
DISEASE:Colorectal cancer (CRC) [MIM:114500]: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (a
Similarity:Belongs to the beta-catenin family.
Contains 12 ARM repeats.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.