Mouse Anti-HSC70 antibody |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF |
推荐稀释比例 |
WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
肿瘤,细胞生物,免疫学,信号转导,转录调节因子 |
标签 |
Array |
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Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Stomach (Mouse) Lysate at 40 ug
Lane 4: Pancreas (Mouse) Lysate at 40 ug
Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 6: Testis (Rat) Lysate at 40 ug
Lane 7: Stomach (Rat) Lysate at 40 ug
Lane 8: Pancreas (Rat) Lysate at 40 ug
Lane 9: 293T (Human) Cell Lysate at 30 ug
Lane 10: MCF-7 (Human) Cell Lysate at 30 ug
Lane 11: Hela (Human) Cell Lysate at 30 ug
Lane 12: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-HSC70 (bsm-33211M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 68 kD
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (ascites of bsm-33211M 8C7) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (ascites of bsm-33211M 8C7) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
RRID:RRID
产品名称:Mouse Anti-HSC70 antibody
别名: 2410008N15Rik; Constitutive heat shock protein 70; Heat shock 70 kDa protein 8; Heat shock 70kD protein 10; Heat shock 70kD protein 8; Heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; Heat shock cognate protein 54; Heat shock cognate protein
中文名称:热休克蛋白71单克隆抗体
英文名称:Mouse Anti-HSC70 antibody
抗体来源: Mouse
克隆类型:单克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein G
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:Recombinant human HSC70 Protein
SWISS:P11142
Gene ID :3312
Human Gene ID:3312
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function:Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
Subunit:Interacts with HSPH1/HSP105. Interacts with IRAK1BP1 (By similarity). Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1,
Subcellular Location:Cytoplasm. Melanosome.
Tissue Specificity:Ubiquitous.
Post-translational modifications:Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated.
Similarity:Belongs to the heat shock protein 70 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.