Mouse Anti-HSP90 beta antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Mouse Anti-HSP90 beta antibody

单克隆 | CloneNo.: 2A2 | SKU：bsm-33136M

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bsm-33136M

￥1180.00-3200.00

50ul

100ul

200ul

货期：现货

售后保障

Mouse Anti-HSP90 beta antibody
产品应用（已验证）	WB
推荐稀释比例	WB=1:500-1000,
研究领域	肿瘤,免疫学,信号转导,转录调节因子
标签	Array

Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: U2os (Human) Cell Lysate at 30 ug
Lane 7: A549 (Human) Cell Lysate at 30 ug
Lane 8: HL60 (Human) Cell Lysate at 30 ug
Lane 9: Jurkat (Human) Cell Lysate at 30 ug
Lane 10: Testis (Mouse) Lysate at 40 ug
Lane 11: Testis (Rat) Lysate at 40 ug
Lane 12: Cerebrum (Mouse) Lysate at 40 ug
Lane 13: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 86 kD
Sample:
Hela KO HSP90 beta(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse t 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
Sample:
Hela (Human) Lysate at 30 ug
Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
Sample:
K562 (Human) Lysate at 30 ug
Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

产品信息

RRID：RRID
产品名称：Mouse Anti-HSP90 beta antibody
别名： HSP90 beta; D6S182; FLJ26984; Heat shock 90kD protein 1, beta; Heat shock protein 90kDa alpha (cytosolic) class B member 1; Heat shock protein beta; Heat shock protein HSP 90 beta; HSP 84; HSP84; HSP 90; HSP-90 BETA; HSP90B; HSPC2; HSPCB; HS90B_HUMAN; Hea
中文名称：热休克蛋白90β/HSP90 β 单克隆抗体
英文名称：Mouse Anti-HSP90 beta antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human HSP90 beta
SWISS：P08238
Gene ID ：3326
Human Gene ID：3326

产品介绍

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012].
Function：Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activ
Subunit：Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4.
Subcellular Location：Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Post-translational modifications：Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity (Probable).
Similarity：Belongs to the heat shock protein 90 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.