| Mouse Anti-alpha Tubulin antibody |
| 反应物种（预测） |
Rat,Chicken,Dog,Pig,Rabbit,GuineaPig |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-1000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
细胞生物,信号转导,细胞骨架, |
| 标签 |
Array |
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Sample:
Lane 1: Human Hela cell Lysates
Lane 2: Human Jurkat cell Lysates
Lane 3: Human SH-SY5Y cell Lysates
Lane 4: Human U-2 OS cell Lysates
Lane 5: Human A431 cell Lysates
Lane 6: Human A549 cell Lysates
Primary: Anti-Alpha Tubulin (bsm-33039M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50kDa
Observed band size: 50kDa
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Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha Tubulin) Monoclonal Antibody, Unconjugated (ascites of bsm-33039M 10F11 ) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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Sample:
Lung (Mouse) Lysate at 40 ug
Hela(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- alpha Tubulin (bsm-33039M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 53 kD
RRID：RRID
产品名称：Mouse Anti-alpha Tubulin antibody
别名： Alpha tubulin 1; Alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alpha 1; Tubulin alpha 1 chain
中文名称：微管蛋白α/Tubulin α/α-tubulin单克隆抗体
英文名称：Mouse Anti-alpha Tubulin antibody
中文别名：α tubulin;
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：Recombinant human alpha Tubulin Protein
SWISS：P68366
Gene ID ：7277
Human Gene ID：7277
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
Function：Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
Subunit：Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtub
Subcellular Location：Cytoplasm, cytoskeleton
Tissue Specificity：Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex
Post-translational modifications：Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of fu
Similarity：Belongs to the tubulin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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