| Mouse Anti-GAP43 antibody |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,神经生物学 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (bs-33192M-8A8) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (bs-33192M-8A8) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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Sample:
BV2 (Mouse) Lysate at 40 ug
VSC4.1(Rat) Lysate at 40 ug
Primary: Anti-GAP43 (bsm-33192M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
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Sample:
Cerebrum(Rat) Lysate at 40 ug
Cerebellum(Rat) Lysate at 40 ug
Primary: Anti-GAP43 (bsm-33192M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 43-46 kD
Observed band size: 43 kD
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Sample:
Spinal cord(Mouse) Lysate at 40 ug
Spinal cord(Rat) Lysate at 40 ug
Primary: Anti-GAP43 (bsm-33192M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 43-46 kD
Observed band size: 43 kD
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Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (bsm-33192M ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
RRID：RRID
产品名称：Mouse Anti-GAP43 antibody
别名： Growth Associated Protein-43; Neuromodulin; Axonal membrane protein GAP 43; B-50; F1; GAP 43; Growth Associated Protein 43; Nerve Growth Related Peptide; Neural phosphoprotein B 50; Neuromodulin; GAP-43; pp46; NEUM_HUMAN; Protein F1; QtrA-11580; QtrA-1307
中文名称：神经生长相关蛋白43单克隆抗体
英文名称：Mouse Anti-GAP43 antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞浆,细胞膜,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：Recombinant human GAP43 Protein
SWISS：P17677
Gene ID ：2596
Human Gene ID：2596
The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function：This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Subunit：Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.
Subcellular Location：Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Note=Cytoplasmic
Post-translational modifications：Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic f
Similarity：Belongs to the neuromodulin family.
Contains 1 IQ domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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