| Mouse Anti-GAPDH-Loading Control antibody |
| 反应物种（预测） |
Chicken,Dog,Rabbit,Sheep,Monkey |
| 产品应用（已验证） |
WB,IHC,ICC |
| 推荐稀释比例 |
WB=1:10000-1:100000,IHC-P=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,新陈代谢, |
| 标签 |
Array |
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Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH-Loading Control) monoclonal Antibody, Unconjugated (bsm-33033M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary:
Anti-GAPDH (bsm-33033M) at 1/2000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 36 kD
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Sample:
Hela (Human) Lysate at 40 ug
Primary:
Anti-GAPDH (bsm-33033M) at 1/2000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 36 kD
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Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Sample:
Lane1: Skin (Mouse) Lysate at 40 ug
Lane2: Testis (Mouse) Lysate at 40 ug
Lane3: Adrenal gland (Mouse) Lysate at 40 ug
Lane4: Lung (Rat) Lysate at 30 ug
Primary: Anti-GAPDH (bsm-33033M) at 1/1 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
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Sample:
Lane 1: Human HEK293 cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Mouse NIH/3T3 cell lysates
Lane 5: Hamster CHO cell lysates
Lane 6: Rat Brain tissue lysates
Primary: Anti-GAPDH (bsm-33033M) at 1/200000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
RRID：RRID
产品名称：Mouse Anti-GAPDH-Loading Control antibody
别名： 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogen
中文名称：3-磷酸甘油醛脱氢酶（内参）单克隆抗体
英文名称：Mouse Anti-GAPDH-Loading Control antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：Recombinded Human GAPDH
SWISS：P04406
Gene ID ：2597
Human Gene ID：2597
Loading Control
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.
Function：Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. N
Subunit：Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear transl
Subcellular Location：Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.
Post-translational modifications：S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated (Probable).
Sulfhydration at Cys-152 increases catalytic activity.
Similarity：Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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