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Mouse Anti-His tag antibody
单克隆  |   CloneNo.: 6E6  |   SKU:bsm-33004M

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货号:bs-3351R
¥1280
订购号:bsm-33004M
¥680.00-4000.00
货期:现货
Mouse Anti-His tag antibody
反应物种(预测)

Species independent

产品应用(已验证)

WB

产品应用(可尝试)

IP

推荐稀释比例

WB=1:5000-50000,IP=1:200-1000,

研究领域

细胞生物,免疫学

标签

Array

  • Sample:
    Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
    Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
    Primary: Anti-His-tag (bsm-33004M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 51 kD
  • Sample:
    Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (bs-41230P) at 10ug
    Primary: Anti-His tag (bsm-33004M) at 1/200000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 41 kDa
    Observed band size: 45 kDa
  • Sample:
    His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2 ug
    Primary: Anti-His tag (bsm-33004M) at 1/2000 ~ 1/10000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 51 kD
  • Sample:
    His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4 ug
    Primary: Anti-His tag (bsm-33004M) at 1/2000 ~ 1/10000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 51 kD
    Observed band size: 51 kD
  • Sample:
    Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug
    Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug
    Primary: Anti-His tag (bsm-33004M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 41 kD
    Observed band size: 45 kD
  • Sample:
    Lane1:Recombinant JSRV-ENV Protein (His tag) at 100 ng
    Lane2:Recombinant JSRV-ENV Protein (His tag) at 100 ng
    Primary: Anti-His tag (bsm-33004M) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
    Predicted band size: 35-45 kD
    Observed band size: 40 kD

产品信息

RRID:RRID
产品名称:Mouse Anti-His tag antibody
别名: HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
中文名称:聚组氨酸抗体标签单克隆抗体
英文名称:Mouse Anti-His tag antibody
抗体来源: Mouse
克隆类型:单克隆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein G
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated HHHHHH peptide
Human Gene ID:His tag

产品介绍

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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