| Mouse Anti-Beta tubulin (Loading Control) antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig,Monkey,Insect, Yeast |
| 产品应用（已验证） |
WB,IHC,ICC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:5000-20000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-500,ICC=1:100, |
| 研究领域 |
细胞骨架,细胞外基质, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of bsm-33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Sample:
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Lovo(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD
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Sample:
A431(Human) Cell Lysate at 30 ug
SY5Y(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD
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Sample:
HL60(Human) Cell Lysate at 30 ug
U937(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
SH-SY5Y(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beta tubulin) monoclonal Antibody, Unconjugated (bsm-33034M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary:
Anti-Beta tubulin (bsm-33034M) at 1/1000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary:
Anti-Beta tubulin (bsm-33034M) at 1/1000~20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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Sample:
Hela (Human) Lysate at 40 ug
Primary:
Anti-Beta tubulin (bsm-33034M) at 1/2000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (ascites of bsm-33034M 7G3) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (ascites of bsm-33034M 7G3) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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Sample:
U87MG Cell (Human) Lysate at 40 ug
SW480 Cell (Human) Lysate at 40 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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Sample:
HL60(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin) Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
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Sample:
Hela(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
SH-SY5Y(Human) Cell Lysate at 30 ug
Cerebellum(Mouse) Lysate at 40 ug
Cerebellum(Rat) Lysate at 40 ug
Cerebrum(Mouse) Lysate at 40 ug
Cerebrum(Rat) Lysate at 40 ug
Primary: Anti-Beta tubulin (bsm-33034M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
RRID：RRID
产品名称：Mouse Anti-Beta tubulin (Loading Control) antibody
别名： Beta 4 tubulin;
Beta 5 tubulin;
beta Ib tubulin;
Beta1 tubulin;
Class I beta tubulin;
M40;
MGC117247;
MGC16435;
OK/SW cl.56;
OK/SWcl.56;
TBB5_HUMAN;
TUBB 1;
TUBB 2;
TUBB 5;
TUBB;
TUBB1;
TUBB2;
TUBB5;
tubulin beta 1 chain;
Tubulin beta 2
中文名称：微管蛋白β tubulin/Tubulin β（内参）单克隆抗体
英文名称：Mouse Anti-Beta tubulin (Loading Control) antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG1
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Beta tubulin
SWISS：P07437
Gene ID ：203068
Human Gene ID：203068
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
Function：Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subunit：Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1.
Subcellular Location：Cytoplasmic, cytoskeleton.
Tissue Specificity：Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Post-translational modifications：Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of fu
Similarity：Belongs to the tubulin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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