| Mouse Anti-beta-Actin (Loading Control) antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Sheep,Fish,GuineaPig,Hamster,Cat |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:5000-20000,Elisa=1:5000-10000,IHC-P=1:200-1000,IHC-F=1:200-1000,Flow Cyt=1ug/Test,IF=1:200-500,ICC=1:100, |
| 研究领域 |
肿瘤,细胞生物,信号转导,细胞骨架, |
| 标签 |
Array |
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Sample:
293T Cell (Human) Lysate at 40 ug
NIH/3T3 Cell (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-β-Actin (bsm-33036M) at 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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Blank control:Hela.
Primary Antibody (green line): Mouse Anti-beta-Actin antibody (bsm-33036M)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-Mouse IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 ug
Hela (Human) Cell Lysate at 30 ug
A549 (Human) Cell Lysate at 30 ug
Primary: Anti-beta-Actin (bsm-33036M) at 1/1000~1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 3: Lung (Rat) Lysate at 40 ug
Lane 4: Lung (Mouse) Lysate at 40 ug
Lane 5: MCF-7 (Human) Cell Lysate at 30 ug
Lane 6: A431 (Human) Cell Lysate at 30 ug
Lane 7: Stomach (Rat) Lysate at 40 ug
Lane 8: Stomach (Mouse) Lysate at 40 ug
Lane 9: Cerebrum (Rat) Lysate at 40 ug
Lane 10: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-beta-Actin (bsm-33036M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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Sample:
Lovo(Human) Cell Lysate at 30 ug
Primary: Anti- beta-Actin (bsm-33036M) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 46 kD
RRID：RRID
产品名称：Mouse Anti-beta-Actin (Loading Control) antibody
别名： Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.
中文名称：β-肌动蛋白/β-Actin（内参）单克隆抗体
英文名称：Mouse Anti-beta-Actin (Loading Control) antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：Synthetic MAP peptide derived from human beta-Actin
SWISS：P60709
Gene ID ：60
Human Gene ID：60
Loading Control
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].
Function：Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit：Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B
Subcellular Location：Cytoplasm. cytoskeleton.
Tissue Specificity：Ubiquitously expressed in all eukaryotic cells.
Post-translational modifications：ISGylated.
Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxi
DISEASE：Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structu
Similarity：Belongs to the actin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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