Rabbit Anti-Phospho-PERK (Thr980) antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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磷酸化抗体

Rabbit Anti-Phospho-PERK (Thr980) antibody

多克隆 | SKU：bs-23340R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-23340R

￥1280.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-Phospho-PERK (Thr980) antibody
反应物种（预测）	Rat,Rabbit
产品应用（已验证）	IHC,ICC
产品应用（可尝试）	IF,ELISA
推荐稀释比例	Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100,
研究领域	肿瘤,细胞生物,免疫学,信号转导,细胞凋亡,转录调节因子,激酶和磷酸酶
标签	Array

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PERK(Thr980)) Polyclonal Antibody, Unconjugated (bs-23340R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-PERK (Thr980)) polyclonal Antibody, Unconjugated (bs-23340R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

产品信息

RRID：RRID
产品名称：Rabbit Anti-Phospho-PERK (Thr980) antibody
别名： p-PERK(Thr980); PERK(Phospho Thr980); PERK(Phospho T980); Phospho-PERK(Thr982)(human); HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation facto
中文名称：磷酸化蛋白激酶样内质网激酶抗体
英文名称：Rabbit Anti-Phospho-PERK (Thr980) antibody
中文别名：p-PERK(Thr982); PERK(Phospho Thr982); PERK(Phospho T982);
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthesised phosphopeptide derived from human PERK around the phosphorylation site of Thr980
抗原表位：H(p-T)GQ
SWISS：Q9Z2B5
Gene ID ：13666
Human Gene ID：9451

产品介绍

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010].
Function：Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfol
Subunit：Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3.
Subcellular Location：Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Tissue Specificity：Ubiquitous.
Post-translational modifications：Autophosphorylated.
N-glycosylated.
Similarity：Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.