Rabbit Anti-phospho-eIF4E (Ser209) antibody |
反应物种(预测) |
Chicken,Dog,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
ICC,IF |
推荐稀释比例 |
WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC=1:50-200, |
研究领域 |
肿瘤,免疫学,信号转导,细胞凋亡 |
标签 |
Array |
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Sample:
Lane 1: Pancreas (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Rat) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Pancreas (Rat) Lysate at 40 ug
Lane 5: Hela (Human) Cell Lysate at 30 ug
Lane 6: 293T (Human) Cell Lysate at 30 ug
Lane 7: MCF-7 (Human) Cell Lysate at 30 ug
Lane 8: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary:
Anti-phospho-eIF4E (Ser209) (bsm-52152R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 25 kD
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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-eIF4E (Ser209)) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-eIF4E (Ser209)) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:AB_2891007
产品名称:Rabbit Anti-phospho-eIF4E (Ser209) antibody
别名: eIF4E(S209); eIF4E(phospho S209); eIF4E(phospho Ser209); p-eIF4E(Ser209); EIF4F; eIF-4E; eIF 4E; CBP; eIF 4F 25 kDa subunit; CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF 4F; EIF4E1; EIF4EL1; EIF4F; Eukaryotic Translation Initiation Factor 4 E; Eukaryotic trans
中文名称:磷酸化真核翻译起始因子4E重组兔单克隆抗体
英文名称:Rabbit Anti-phospho-eIF4E (Ser209) antibody
抗体来源: Rabbit
克隆类型:单克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209
抗原表位:SG(p-S)TT
SWISS:P06730
Gene ID :1977
Human Gene ID:1977
eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
Function:Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducin
Subunit:eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF
Subcellular Location:Cytoplasm, P-body.
Post-translational modifications:Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Similarity:Belongs to the eukaryotic initiation factor 4E family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.