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磷酸化抗体 重组兔单抗
Rabbit Anti-phospho-IKB alpha (Ser32) antibody
单克隆  |   CloneNo.: 13E7  |   SKU:bsm-52169R

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货号:bs-3351R
¥1280
订购号:bsm-52169R
¥1580.00-2680.00
货期:现货
Rabbit Anti-phospho-IKB alpha (Ser32) antibody
反应物种(预测)

Mouse,Rat,Pig,Cow,Rabbit,Sheep

产品应用(已验证)

WB,ICC,FCM

产品应用(可尝试)

IHC,IF

推荐稀释比例

WB=1:500-1000,IHC-P=1:50-200,IHC-F=1:50-200,Flow Cyt=2ug/Test,IF=1:50-200,ICC=1:50,

研究领域

肿瘤,细胞生物,神经生物学,信号转导,激酶和磷酸酶

标签

Array

  • Blank control:MCF7.
    Primary Antibody (green line): Rabbit Anti-phospho-IKB alpha (Ser32) antibody (bsm-52169R)
    Dilution: 2μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-AF488
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
  • Sample:
    Lane 1: SK-Br-3 cell lysates
    Primary: Anti-phospho-IKB alpha (Ser32) (bsm-52169R) at 1/500 dilution
    Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
    Predicted band size: 36 kD
    Observed band size: 36 kD
  • Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-IKB alpha(S32)) monoclonal Antibody, Unconjugated (bsm-52169R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

产品信息

RRID:RRID
产品名称:Rabbit Anti-phospho-IKB alpha (Ser32) antibody
别名: IKB alpha(S32); IKB alpha (phospho S32); IKB alpha (phospho Ser32); p-IKB alpha (phospho S32); I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; IKBA_HUMAN; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer-binding p
中文名称:磷酸化核因子κB抑制蛋白α重组兔单克隆抗体
英文名称:Rabbit Anti-phospho-IKB alpha (Ser32) antibody
抗体来源: Rabbit
克隆类型:单克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human IKB alpha around the phosphorylation site of Ser32
抗原表位:D(p-S)GL
SWISS:P25963
Gene ID :4792
Human Gene ID:4792

产品介绍

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
Function:Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiq
Subcellular Location:Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Post-translational modifications:Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. Sumoylated; sumoylation requires the presence
DISEASE:Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectoderma
Similarity:Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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