Rabbit Anti-phospho-P53 (Ser392) antibody |
反应物种(预测) |
Dog,Pig,Cow,Horse,Sheep |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,ICC,IF |
推荐稀释比例 |
WB=1:1000-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC=1:50-200, |
研究领域 |
肿瘤,细胞生物,信号转导,细胞凋亡,转录调节因子 |
标签 |
Array |
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Sample:
Lane 1: Urinary bladder (Mouse) Lysate at 40 ug
Lane 2: Esophagus (Rat) Lysate at 40 ug
Lane 3: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-P53 (Ser392) (bsm-52182R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
RRID:RRID
产品名称:Rabbit Anti-phospho-P53 (Ser392) antibody
别名: p53(S392); P53 (phospho S392); P53 (phospho Ser392); p-P53 (Ser392); p-P53 (S392); Widespread p53; Wtp53; Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; HGNC11998; LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53
中文名称:磷酸化肿瘤抑制基因P53重组兔单克隆抗体
英文名称:Rabbit Anti-phospho-P53 (Ser392) antibody
抗体来源: Rabbit
克隆类型:单克隆
细胞定位:细胞核,细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human p53 around the phosphorylation site of Ser392
抗原表位:PD(p-S)D
SWISS:P04637
Gene ID :7157
Human Gene ID:7157
p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.
p53 can apparently be phosphorylated by ATM, ATR, and DNAPK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. p53 is phosphorylated at Ser6 and Ser9 by ck1d and ck1e both in vitro and in vivo. Phosphorylation of p53 at Ser46 is important in regulating the ability of p53 to induce apoptosis. In vivo phosphorylation of p53 at Ser33 by cdk7/cyclin H and in response to UV irradiation has been observed.
Function:This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arr
Subunit:Interacts with AXIN1. Probably part of a complex consisting of TP53, HIPK2 and AXIN1. Binds DNA as a homotetramer. Interacts with histone acetyltransferases EP300 and methyltransferases HRMT1L2 and CARM1, and recruits them to promoters. In vitro, the inte
Subcellular Location:Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Note=Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2.
Tissue Specificity:Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3
Post-translational modifications:Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence.
Phosphorylation on Ser residues mediates transcriptional
DISEASE:Note=TP53 is found in increased amounts in a wide variety of transformed cells. TP53 is frequently mutated or inactivated in about 60% of cancers. TP53 defects are found in Barrett metaplasia a condition in which the normally stratified squamous epitheliu
Similarity:Belongs to the p53 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.