| Rabbit Anti-Phospho-YAP1 (Ser127) antibody |
| 反应物种（预测） |
Mouse,Rat |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,ICC,IF |
| 推荐稀释比例 |
WB=1:500-1000,IHC-P=1:50-200,IHC-F=1:50-200,Flow Cyt=2ug/Test,IF=1:50-200,ICC=1:50-200, |
| 研究领域 |
肿瘤,细胞生物,免疫学,信号转导,干细胞,细胞凋亡,转录调节因子 |
| 标签 |
Array |
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Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-YAP1 (Ser127) (bsm-52214R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65-70 kD
Observed band size: 70 kD
-
Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-Phospho-YAP1 (Ser127) antibody (bsm-52214R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-Phospho-YAP1 (Ser127) antibody
别名： Phospho-YAP1 (S127); YAP1 (S127); YAP1 (Phospho Ser127); YAP1 (Phospho S127); 65 kDa Yes associated protein; YAp 1; YAP 65; YAP; YAP2; YAP65; Yes associated protein 1 65kDa; Yes associated protein 1; Yes associated protein 2; YKI; Transcriptional coactiva
中文名称：磷酸化原癌基因Yes相关蛋白1重组兔单克隆抗体
英文名称：Rabbit Anti-Phospho-YAP1 (Ser127) antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human YAP around the phosphorylation site of Ser127
抗原表位：AH(p-S)SP
SWISS：P46937
Gene ID ：10413
Human Gene ID：10413
This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq].
Function：Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferat
Subunit：Binds to the SH3 domain of the YES kinase. Binds to WBP1 and WBP2. Binds, in vitro, through the WW1 domain, to neural isoforms of ENAH that contain the PPSY motif (By similarity). The phosphorylated form interacts with YWHAB. Interacts (via WW domains) wi
Subcellular Location：Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated
Tissue Specificity：Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
Post-translational modifications：Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
Similarity：Belongs to the YORKIE family.
Contains 2 WW domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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