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Rabbit Anti-Integrin alpha 2 antibody
单克隆  |   CloneNo.: 14D7  |   SKU:bsm-52613R

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货号:bs-3351R
¥1280
订购号:bsm-52613R
¥1580.00-2680.00
货期:现货
Rabbit Anti-Integrin alpha 2 antibody
产品应用(已验证)

WB,IHC

产品应用(可尝试)

IF

推荐稀释比例

WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,

研究领域

肿瘤,细胞生物,细胞粘附分子,

标签

Array

  • Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • Paraformaldehyde-fixed, paraffin embedded (human skin carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • Sample:
    Lane 1: Kidney (Mouse) Lysate at 40 ug
    Lane 2: Large intestine (Mouse) Lysate at 40 ug
    Lane 3: Stomach (Mouse) Lysate at 40 ug
    Lane 4: Skin (Mouse) Lysate at 40 ug
    Lane 5: Kidney (Rat) Lysate at 40 ug
    Lane 6: Large intestine (Rat) Lysate at 40 ug
    Lane 7: Stomach (Rat) Lysate at 40 ug
    Lane 8: Skin (Rat) Lysate at 40 ug
    Lane 9: HT1080 (Human) Cell Lysate at 30 ug
    Lane 10: MCF-7 (Human) Cell Lysate at 30 ug
    Lane 11: Huvec (Human) Cell Lysate at 30 ug
    Primary: Anti-Integrin alpha 2 (bsm-52613R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 140 kD
    Observed band size: 140 kD

产品信息

RRID:RRID
产品名称:Rabbit Anti-Integrin alpha 2 antibody
别名: CD49b antigen; BR; CD 49b; CD49b; CD49 antigen like family member B; Collagen receptor; DX5; Glycoprotein Ia deficiency included; GP Ia; GP Ia deficiency, included; GPIa; HPA 5 included; HPA5 included; Itga2; Platelet alloantigen Br(a), included; Platelet
中文名称:整合素α2(CD49b)重组兔单克隆抗体
英文名称:Rabbit Anti-Integrin alpha 2 antibody
抗体来源: Rabbit
克隆类型:单克隆
细胞定位:细胞膜
性 状:Lyophilized or Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

免疫原信息

免 疫 原:Recombinant human Integrin alpha 2
SWISS:P17301
Gene ID :3673
Human Gene ID:3673

产品介绍

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Function:Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collag
Subunit:Heterodimer of an alpha and a beta subunit. Alpha-2 associates with beta-1. Interacts with HPS5 and RAB21. Binds to human echoviruses 1 and 8 capsid proteins and acts as a receptor for these viruses. Interacts (via ITAG2 I-domain) with rotavirus A VP4; th
Subcellular Location:Membrane; Single-pass type I membrane protein.
Similarity:Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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