| Rabbit Anti-SCD antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
WB,IHC,ICC |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:50-100,IHC-F=1:50-100,IF=1:50-100,ICC=1:100, |
| 研究领域 |
肿瘤,心血管,免疫学,信号转导,合成与降解,脂蛋白,新陈代谢, |
| 标签 |
Array |
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Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Primary:
Anti-SCD (bs-55193R) at 1/1000 dilution
Secondary:
IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
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U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)SCD polyclonal Antibody, Unconjugated (bs-55193R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD) Polyclonal Antibody, Unconjugated (bs-55193R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: Hela (Human) Cell Lysate at 25 ug
Lane 2: SCD knockout (KO) Hela (Human) Cell Lysate at 25 ug
Primary: Anti-RHOC (bs-55193R) at 1/1000 dilution
Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD) Polyclonal Antibody, Unconjugated (bs-55193R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：RRID
产品名称：Rabbit Anti-SCD antibody
别名： ACOD_HUMAN; Acyl-CoA desaturase; Delta(9)-desaturase; Delta-9 desaturase; Delta-9-Desaturase; FADS5; Fatty acid desaturase; PRO0998; SCD 1; SCD; SCD1; Stearoyl-CoA Desaturase.
中文名称：固醇酰辅酶A脱氢酶1抗体
英文名称：Rabbit Anti-SCD antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
免 疫 原：KLH conjugated synthetic peptide derived from human SCD
抗原表位：200-359/359
SWISS：O00767
Gene ID ：6319
Human Gene ID：6319
Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme is constitutively expressed in adipose tissue and is induced in liver in response to a fat free, high carbohydrate diet. SCD1 expression is higher in female than male mouse. The repression of RNA levels and enzyme activity of hepatic SCD1 by Leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD1 could be of benefit for treatment of obesity, hepatic steatosis and other metabolic disorders.
Function：Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stear
Subcellular Location：Endoplasmic reticulum membrane; Multi-pass membrane protein (Probable).
Similarity：Belongs to the fatty acid desaturase family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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