Rabbit Anti-Human IgM antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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重组兔单抗

Rabbit Anti-Human IgM antibody

单克隆 | CloneNo.: 4C2 | SKU：bsm-52860R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bsm-52860R

￥1580.00-2680.00

50ul

100ul

货期：现货

售后保障

Rabbit Anti-Human IgM antibody
产品应用（已验证）	WB,IHC
产品应用（可尝试）	IP
推荐稀释比例	WB=1:1000-5000,IP=1:20-50,IHC-P=1:50-200,IHC-F=1:50-200,
标签	Array

Paraformaldehyde-fixed, paraffin embedded (human spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Human IgM) Monoclonal Antibody, Unconjugated (bsm-52860R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Human IgM) Monoclonal Antibody, Unconjugated (bsm-52860R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Lane 1: human plasma
Primary: Anti-Human IgM (bsm-52860R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 900 kD
Observed band size: 75 kD
Sample:
Lane 1: Human Serum
Primary: Anti- Human IgM (bsm-52860R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 900 kDa
Observed band size: 75 kDa
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Human IgM) Monoclonal Antibody, Unconjugated (bsm-52860R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

产品信息

RRID：RRID
产品名称：Rabbit Anti-Human IgM antibody
别名： human Immunoglobulin M
中文名称：人IgM重组兔单克隆抗体
英文名称：Rabbit Anti-Human IgM antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：分泌型蛋白
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant

免疫原信息

免疫原：Human IgM
Human Gene ID：human IgM

产品介绍

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.