| Rabbit Anti-CD9 antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ICC,ELISA |
| 推荐稀释比例 |
WB=1:500-1000,Elisa=1:5000-10000,IHC-P=1:50-200,IHC-F=1:50-200,ICC=1:100, |
| 研究领域 |
肿瘤,细胞生物,免疫学,发育生物学,细胞表面分子, |
| 标签 |
Array |
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Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: Heart (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Mouse) Lysate at 40 ug
Lane 5: Huvec (Human) Cell Lysate at 30 ug
Lane 6: A431 (Human) Cell Lysate at 30 ug
Lane 7: SW480 (Human) Cell Lysate at 30 ug
Primary: Anti-CD9 (bsm-52264R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 24 kD
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Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：RRID
产品名称：Rabbit Anti-CD9 antibody
别名： Tetraspanin 29; 5H9; 5H9 antigen; Antigen defined by monoclonal; 602 29; Antigen defined by monoclonal; 60229; BA 2; BA2; BTCC 1; BTCC1; CD 9; CD9; CD9 antigen; CD9 antigen p24; CD9 molecule; CD9_HUMAN; Cell growth inhibiting gene 2 protein; Cell growth-i
中文名称：CD9重组兔单克隆抗体
英文名称：Rabbit Anti-CD9 antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
免 疫 原：Recombinant human CD9
抗原表位：111-196/228
抗原细胞定位：Extracellular
SWISS：P21926
Gene ID ：928
Human Gene ID：928
CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve.
Function：Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Subunit：Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8.
Subcellular Location：Membrane; Multi-pass membrane protein.
Tissue Specificity：Expressed by a variety of hematopoietic and epithelial cells.
Post-translational modifications：Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
Similarity：Belongs to the tetraspanin (TM4SF) family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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