| Rabbit Anti-mTOR antibody |
| 产品应用（已验证） |
WB |
| 推荐稀释比例 |
WB=1:500-1000, |
| 研究领域 |
肿瘤,心血管,细胞生物,免疫学,染色质和核信号,细胞周期蛋白,转录调节因子,表观遗传学, |
| 标签 |
Array |
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Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: K562 (Human) Cell Lysate at 30 ug
Lane 6: Siha (Human) Cell Lysate at 30 ug
Primary: Anti-mTOR (bsm-54471R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 290 kD
Observed band size: 270 kD
RRID：RRID
产品名称：Rabbit Anti-mTOR antibody
别名： FKBP12; dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1); FK506 binding protein 12 rapamycin associated protein 1; FK506 binding protein 12 rapamycin associated protein 2; FK506 binding protein 12 rapamycin complex associated protein 1;
中文名称：雷帕霉素靶蛋白重组兔单克隆抗体
英文名称：Rabbit Anti-mTOR antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
免 疫 原：KLH conjugated synthetic peptide derived from human mTOR
抗原表位：2350-2440/2549
SWISS：P42345
Gene ID ：2475
Human Gene ID：2475
mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 289-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.
Function：Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. Functions as part of 2 structurally and functionally distinct signaling
Subunit：Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Interacts
Subcellular Location：Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome. Cytoplasm (By si
Tissue Specificity：Expressed in numerous tissues, with highest levels in testis.
Post-translational modifications：Phosphorylated. Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261 promotes autophosphorylation.
Similarity：Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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