Rabbit Anti-C-Myc antibody |
反应物种(预测) |
Fusion Protein |
产品应用(已验证) |
WB |
推荐稀释比例 |
WB=1:500-2000, |
研究领域 |
肿瘤,细胞生物,信号转导,干细胞,生长因子和激素,转录调节因子,激酶和磷酸酶,表观遗传学, |
标签 |
Array |
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Sample:
Lane 1: Raji (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: Jurkat (Human) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Lane 5: Molt-4 (Human) Cell Lysate at 30 ug
Lane 6: U937 (Human) Cell Lysate at 30 ug
Lane 7: HL60 (Human) Cell Lysate at 30 ug
Lane 8: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-C-Myc (bs-24507R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 60 kD
RRID:RRID
产品名称:Rabbit Anti-C-Myc antibody
别名: AU016757; Avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; Cellular myelocytomatosis oncogene; MGC105490; MRTL; Myc protein; Myc proto oncogene protein; Myc-related translation/localization regulatory factor; Myc2; myca; Myelocytomatosis onc
中文名称:致癌基因C-Myc抗体
英文名称:Rabbit Anti-C-Myc antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human C-Myc
抗原表位:271-370/438
SWISS:P01106
Gene ID :4609
Human Gene ID:4609
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008].
Function:Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Subunit:Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FB
Subcellular Location:Nucleus, nucleoplasm. Nucleus, nucleolus.
Post-translational modifications:Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the
DISEASE:Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
D
Similarity:Contains 1 basic helix-loop-helix (bHLH) domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.