| Rabbit Anti-Ephrin B2 antibody |
| 反应物种（预测） |
Mouse |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
WB=1:500-1000,IHC-P=1:400-800,IF=1:50-200, |
| 研究领域 |
细胞生物,神经生物学,信号转导,激酶和磷酸酶 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (bsm-54090R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (bsm-54090R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (bsm-54090R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ephrin B2) Monoclonal Antibody, Unconjugated (bsm-54090R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-Ephrin B2 (bsm-54090R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45-50 kD
Observed band size: 47 kD
RRID：RRID
产品名称：Rabbit Anti-Ephrin B2 antibody
别名： EFN B2; EFNB 2; Efnb2; EFNB2_HUMAN; Eph related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 5; ephrin B2; Ephrin-B2; EphrinB2; EPLG 5; EPLG5; Htk L; HTK ligand; HTK-L; HTKL; LERK 5; LERK-5; LERK5; Ligand of eph related k
中文名称：Ephrin B2蛋白重组兔单克隆抗体
英文名称：Rabbit Anti-Ephrin B2 antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Ephrin B2
SWISS：P52799
Gene ID ：1948
Human Gene ID：1948
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008]
Function：Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent
Subcellular Location：Membrane.
Tissue Specificity：Lung and kidney.
Post-translational modifications：Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
Similarity：Belongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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