| Rabbit Anti-Cleaved PARP antibody |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ICC |
| 推荐稀释比例 |
WB=1:500-2000,IP=1:20-50,Flow Cyt=1:50,ICC=1:50-200, |
| 研究领域 |
肿瘤,心血管,细胞生物,信号转导,细胞凋亡,新陈代谢,线抗体, |
| 标签 |
Array |
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Sample:
Lane 1: Jurkat cell lysate
Lane 2: A549 cell lysate
Primary: Anti-Cleaved PARP (bsm-52408R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 89/110 kD
Observed band size: 89 kD
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Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Cleaved PARP antibody (bsm-52408R )
Dilution: 1:50;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1:1000.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-Cleaved PARP antibody
别名： ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; PARP-1; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymera
中文名称：多腺苷二磷酸多聚酶/多聚ADP-核糖聚合酶1重组兔单克隆抗体
英文名称：Rabbit Anti-Cleaved PARP antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞核,细胞浆
性 状：Lyophilized or Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：Recombinant human PARP protein
SWISS：P09874
Gene ID ：142
Human Gene ID：142
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].
Function：Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligat
Subunit：Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and Z
Subcellular Location：Mitochondrion outer membrane; Single-pass membrane protein.
Nucleus membrane; Single-pass membrane protein.
Endoplasmic reticulum membrane; Single-pass membrane protein.
Nucleus.
Post-translational modifications：Phosphorylated by PRKDC and TXK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription
Similarity：Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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