| Rabbit Anti-Tissue factor antibody |
| 产品应用（已验证） |
WB,IHC,ICC |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IF=1:100-500,ICC=1:50, |
| 研究领域 |
心血管,细胞生物,免疫学,生长因子和激素,细胞表面分子, |
| 标签 |
Array |
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SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Tissue Factor) monoclonal Antibody, Unconjugated (bsm-52647R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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PANC-1 Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Tissue Factor) monoclonal Antibody, Unconjugated (bsm-52647R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Sample:
Lane 1: mouse brain tissue lysate
Lane 2: U937 cell lysate
Lane 3: SH-SY5Y cell lysate
Primary: Anti-Tissue factor (bsm-52647R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 35 kD
Observed band size: 50 kD
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SHG-44 Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Tissue Factor) monoclonal Antibody, Unconjugated (bsm-52647R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Monoclonal Antibody, Unconjugated (bsm-52647R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Monoclonal Antibody, Unconjugated (bsm-52647R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tissue factor) Monoclonal Antibody, Unconjugated (bsm-52647R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Rat Kidney Lysates
Lane 3: Human A431 cell Lysates
Lane 4: Human MDA-MB-231 cell Lysates
Lane 5: Human U-87 MG cell Lysates
Lane 6: Human PANC-1 cell Lysates
Primary: Anti-Tissue factor (bsm-52647R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35kDa
Observed band size: 45kDa
RRID：RRID
产品名称：Rabbit Anti-Tissue factor antibody
别名： CD 142; CD-142; CD142; CD142 antigen; Coagulation factor III (thromboplastin tissue factor); Coagulation factor III; F3; TF; TFA; Thromboplastin; Tissue factor; TF_HUMAN.
中文名称：组织因子（CD142）兔单克隆抗体
英文名称：Rabbit Anti-Tissue factor antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Tissue factor
抗原表位：1-100/295
SWISS：P13726
Gene ID ：2152
Human Gene ID：2152
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Function：Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagat
Subunit：Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII.
Subcellular Location：Isoform 1: Membrane; Single-pass type I membrane protein.
Isoform 2: Secreted.
Tissue Specificity：Lung, placenta and pancreas.
Similarity：Belongs to the tissue factor family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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