| Rabbit Anti-Histone H3 (acetyl K4) antibody |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000, |
| 研究领域 |
染色质和核信号,细胞类型标志物(Mitosis Marker),表观遗传学, |
| 标签 |
Array |
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Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Primary: Anti-Histone H3 (acetyl K4) (bs-24644R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 17 kD
RRID：RRID
产品名称：Rabbit Anti-Histone H3 (acetyl K4) antibody
别名： H3K4ac; Histone H3(acetyl Lys4); Acetyl-Histone H3(Lys4); Acetyl-Histone H3(K4); H3 histone family member E pseudogene;
H3 histone family, member A;
H3/A;
H31_HUMAN;
H3F3;
H3FA;
Hist1h3a;
HIST1H3B;
HIST1H3C;
HIST1H3D;
HIST1H3E;
HIST1H3F;
HIST1
中文名称：乙酰化组蛋白H3抗体
英文名称：Rabbit Anti-Histone H3 (acetyl K4) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K4
抗原表位：RT(Acetyl-K)QT
SWISS：P68431
Gene ID ：8350
Human Gene ID：8350
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.
Function：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replic
Subunit：The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with GCN5, whereby H3S10ph increases hist
Subcellular Location：Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC).
Post-translational modifications：Phosphorylated to form H3S10ph. H3S10ph promotes subsequent H3K14ac formation by GCN5. H3S10ph is only found in the mitotically dividing MIC, but not in the amitotically dividing MAC. H3S10ph is correlated with chromosome condensation during mitotic or me
Similarity：Belongs to the histone H3 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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