Rabbit Anti-APG4B(Acetyl K39) antibody |
反应物种(预测) |
Pig |
产品应用(已验证) |
IHC |
推荐稀释比例 |
IHC-P=1:400-800, |
研究领域 |
肿瘤,细胞生物,信号转导,细胞凋亡 |
标签 |
Array |
-
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B(Acetyl K39)) Polyclonal Antibody, Unconjugated (bs-2845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B(Acetyl K39)) Polyclonal Antibody, Unconjugated (bs-2845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APG4B(Acetyl K39)) Polyclonal Antibody, Unconjugated (bs-2845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:RRID
产品名称:Rabbit Anti-APG4B(Acetyl K39) antibody
别名: APG4B (Ac-Lys39); APG4B (Acetyl Lys39); APG4B (Ac-K39); APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); AUT like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; C
中文名称:乙酰化自噬相关蛋白4B抗体
英文名称:Rabbit Anti-APG4B(Acetyl K39) antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Lyophilized or Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human APG4B(Acetyl K39)
SWISS:Q9Y4P1
Gene ID :23192
Human Gene ID:23192
ATG4B is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a reveal
Subcellular Location:Cytoplasm (Probable).
Tissue Specificity:Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Similarity:Belongs to the peptidase C54 family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.