Rabbit Anti-EIF2S1 antibody |
反应物种(预测) |
Rat |
产品应用(已验证) |
WB,IHC |
推荐稀释比例 |
WB=1:500-2000,IHC-P=1:50-100, |
研究领域 |
肿瘤,细胞生物,信号转导,细胞凋亡,转录调节因子,激酶和磷酸酶 |
标签 |
Array |
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Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Human Hepg2 cell lysates
Lane 3: Human Mcf-7 cell lysates
Lane 4: Human Hela cell lysates
Lane 5: Human K562 cell lysates
Primary: Anti- EIF2S1 (bsm-60003R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 36-38 kD
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Tissue:Human breast
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60003R
RRID:RRID
产品名称:Rabbit Anti-EIF2S1 antibody
别名: EIF2A; CDA 02; CDA02; eIF 2 alpha; EIF 2; EIF 2A; EIF-2alpha; EIF2; EIF2alpha; eIF2S1; Eukaryotic Translation Initiation Factor 2 alpha; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa; Eukaryotic translation initiation factor 2 subunit a
中文名称:真核启动因子2α(eIFα2)兔单克隆抗体
英文名称:Rabbit Anti-EIF2S1 antibody
抗体来源: Rabbit
克隆类型:单克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human EIF2S1
SWISS:P05198
Gene ID :1965
Human Gene ID:1965
eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis.
Function:Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit
Subunit:Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation. Interacts
Subcellular Location:Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules.
Post-translational modifications:Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to
Similarity:Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.