| Mouse Anti-WNT10B antibody |
| 反应物种（预测） |
Mouse |
| 产品应用（已验证） |
WB,IHC,ICC |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:50-1:100,IF=1:100-500,ICC=1:50, |
| 研究领域 |
肿瘤,免疫学,信号转导,转录调节因子 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT10B) Monoclonal Antibody, Unconjugated (bsm-60528M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60528M
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Tissue: Human liver cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60528M
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Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:10000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: SH-SY5Y, 3: C2C12
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 43 kDa
Observed MW: 43 kDa
RRID：RRID
产品名称：Mouse Anti-WNT10B antibody
别名： WN10B_HUMAN; SHFM6; wingless type MMTV integration site family, member 10B; wnt12; WNT10-B.
中文名称：信号通路WNT10B重组鼠单克隆抗体
英文名称：Mouse Anti-WNT10B antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG1/Kappa
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human WNT10B
SWISS：O00744
Gene ID ：7480
Human Gene ID：7480
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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