| Mouse Anti-FIS1 antibody |
| 反应物种（预测） |
Mouse |
| 产品应用（已验证） |
WB,ICC |
| 产品应用（可尝试） |
IHC,IF |
| 推荐稀释比例 |
WB=1:500-1000,IHC-P=1:20-100,IF=1:20-100,ICC=1:20-100, |
| 研究领域 |
细胞生物,细胞凋亡 |
| 标签 |
Array |
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Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: MCF-7, 2: T47D, 3: MDA-MB-231
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
-
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (FIS1) monoclonal Antibody, Unconjugated (bsm-60551M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
RRID：RRID
产品名称：Mouse Anti-FIS1 antibody
别名： CGI 135; CGI135; FIS 1; FIS1; Fis1 homolog; FIS1, S. cerevisiae, homolog of; FIS1_HUMAN; Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae); Fission 1 (mitochondrial outer membrane) homolog (yeast); Fission 1 (mitochondrial outer membrane) h
中文名称：线粒体裂变1蛋白重组鼠单克隆抗体
英文名称：Mouse Anti-FIS1 antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG1/Kappa
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human FIS1
SWISS：Q9Y3D6
Gene ID ：51024
Human Gene ID：51024
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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