| Mouse Anti-SNAP25 antibody |
| 产品应用（已验证） |
WB,IHC |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:20-100, |
| 研究领域 |
细胞生物,神经生物学,信号转导,生长因子和激素 |
| 标签 |
Array |
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Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1: 2000
Primary ab incubation condition: 2 hours at
room temperature
Lysate: Mouse brain, Rat brain
Protein loading quantity: 20 μg
Exposure time: 15 s
Predicted MW: 23 kDa
Observed MW: 25 kDa
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Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (bsm-60238M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (bsm-60238M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (bsm-60238M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Tissue: Human cerebellum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60238M
RRID：RRID
产品名称：Mouse Anti-SNAP25 antibody
别名： SNAP-25; bA416N4.2; Bdr; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC9; SNAP 25; SNAP; SNAP-25B; SNP 25; SNP25; sp; SUP; Super protein; Synaptosomal associated 25 kDa prote
中文名称：突触相关蛋白25重组鼠单克隆抗体
英文名称：Mouse Anti-SNAP25 antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG1
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human SNAP25
SWISS：P60880
Gene ID ：6616
Human Gene ID：6616
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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