| Rabbit Anti-alpha Actinin 4 antibody |
| 产品应用（已验证） |
WB,IHC,ICC |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:50-1:100,IF=1:100-500,ICC=1:50, |
| 研究领域 |
肿瘤,信号转导,新陈代谢,细胞骨架, |
| 标签 |
Array |
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Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1:HeLa, 2:HEK-293, 3:4T1, 4:Raw264.7
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 100 kDa
Observed MW: 110 kDa
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Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60423R
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Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60423R
RRID：RRID
产品名称：Rabbit Anti-alpha Actinin 4 antibody
别名： actinin 4;
Actinin alpha 4;
Actinin alpha-4;
Alpha-actinin-4;
α-Actinin-4;
αActinin-4;
actinin4;
ACTN 4;
ACTN4;
ACTN4_HUMAN;
DKFZp686K23158;
F actin cross linking protein;
F-actin cross-linking protein;
Focal segmental glomerulosclerosis 1;
中文名称：α-辅肌动蛋白4重组兔单克隆抗体
英文名称：Rabbit Anti-alpha Actinin 4 antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human alpha Actinin 4
SWISS：O43707
Gene ID ：81
Human Gene ID：81
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008].
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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