| Mouse Anti-CD11a antibody |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
ICC,IF |
| 推荐稀释比例 |
Flow Cyt=1ug/Test,IF=1:50,ICC=1:50, |
| 研究领域 |
细胞生物,干细胞,细胞粘附分子, |
| 标签 |
Array |
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Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60413M
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Blank control:Raji.
Primary Antibody (green line): Mouse Anti-CD11a antibody (bsm-60413M)
Dilution: 1:100;
Secondary Antibody : Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Mouse Anti-CD11a antibody
别名： CD11a; Integrin alpha-L; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain; Lymphocyte antigen 15; Ly-15; CD11 antigen-like family member A; CD_antigen; ITAL_HUMAN.
中文名称：整合素αL重组鼠单克隆抗体
英文名称：Mouse Anti-CD11a antibody
抗体来源： Mouse
克隆类型：单克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CD11a
SWISS：P20701
Gene ID ：3683
Human Gene ID：3683
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function：Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes an
Subunit：Heterodimer of an alpha and a beta subunit. Alpha-L associates with beta-2.
Subcellular Location：Membrane; Single-pass type I membrane protein.
Tissue Specificity：Leukocytes.
Similarity：Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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