| Rabbit Anti-Aurora B antibody |
| 产品应用（已验证） |
WB,IHC,ICC |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
WB=1:500-1000,IHC-P=1:50-100,IF=1:50,ICC=1:50, |
| 研究领域 |
肿瘤,细胞生物,神经生物学,信号转导,细胞周期蛋白,激酶和磷酸酶 |
| 标签 |
Array |
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Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at
room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HeLa, 2: 293, 3: A20
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 39 kDa
Observed MW: 39 kDa
-
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60554R
-
Tissue: Mouse spleen
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60554R
-
Cell line: A549
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60554R
-
Sample:
Lane 1: Human Hela cell Lysates
Lane 2: Human 293T cell Lysates
Primary: Anti-Aurora B (bsm-60554R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kDa
Observed band size: 35kDa
RRID：RRID
产品名称：Rabbit Anti-Aurora B antibody
别名： STK-1; serine/threonine kinase 12; aurora-B; aurora-1; aurora kinase B-Sv1; aurora kinase B-Sv2; ARK-2; STK-1; STK1; aurora-related kinase 2; aurora/IPL1-related kinase 2; serine/threonine-protein kinase aurora-B; aurora- and Ipl1-like midbody-associated
中文名称：有丝分裂激酶B重组兔单克隆抗体
英文名称：Rabbit Anti-Aurora B antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein G
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Aurora B
SWISS：Q96GD4
Gene ID ：9212
Human Gene ID：9212
This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromsomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012].
Function：Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and
Subunit：Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Associates with RACGAP1 during M phase. Interacts with CDCA1, EVI5, JTB, NDC80, PSMA3, SEPT1 and TACC1. Interacts with SPDYC
Subcellular Location：Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokines
Tissue Specificity：High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Post-translational modifications：The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity.
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin
DISEASE：Note=Disruptive regulation of expression is a possible mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
Similarity：Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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